The Laboratory is seeking a full-time Research Fellow to investigate the role of cardiolipin (CL) in mtDNA replication and mitochondrial division. This will be achieved by studying CL metabolism and PL trafficking pathways in mutant and knockout TRIAP1 fibroblasts and mutant TRIAP1 human induced pluripotent stem cell (hiPSC)-derived myoblasts. The principal activities will include cell culture (fibroblasts, iPSCs), CRIPR/Cas9, PCR and DNA sequencing (Sanger and NGS), lipidomics and proteomics (SiLAC and BioID), mass spectrometry, imaging techniques (confocal and super-resolution microscopy, live cell imaging and electron microscopy), and molecular and cell biology (Western blot, BN-PAGE, Seahorse, oxygraph, subcellular fractionation, spectrophotometric assays, Southern blot/long PCR).
The candidate will be supervised directly by Dr Robert Pitceathly, but will work as part of a team of researchers who have varying responsibilities within an integrated program at UCL. Dr Julien Prudent will help supervise any work undertaken at the MRC MBU, Cambridge. Consequently, the post holder will develop new skills by working closely with experimental biologists in exciting interdisciplinary environments with world-leading expertise in mitochondrial biology and mitochondrial diseases.
There is an opportunity to gain experience in some laboratory techniques, including aspects of mitochondrial cell biology and analysis of membrane contact sites, at the MRC Mitochondrial Biology Unit (MBU), University of Cambridge.
The successful candidate will have an Honours degree (minimum 2:1) in a related discipline and a PhD in a relevant subject (eg. cellular/molecular biology, neuroscience). Strong knowledge and experience of cell culture, molecular cell biology and microscopy (confocal and super-resolution microscopy) is required.
Please apply via UCL Human Resources – ‘Jobs at UCL’ and the Job Ref is 1791582.
Closing date:2019-04-18Contract length:Fixed-Term Contract – Three yearsInstitution:UCLDepartment:Neuromuscular Diseases, UCL Queen Square Institute of Neurology
Department of Neuromuscular Diseases
UCL Queen Square Institute of Neurology
London WC1N 3BG